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2022
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09
This article shares the functions of the CO2 incubator
CO2 incubators are widely used in the research and production of medicine, immunology, genetics, microbiology, agricultural science and pharmacology, and have become one of the routine instruments commonly used in laboratories in the above fields. Users have two basic requirements for CO2 incubators. One is to provide stable temperature, carbon dioxide concentration and humidity control, thus facilitating the progress of their research work. The second is to require the CO2 incubator to effectively prevent microbial contamination in the incubator, and to eliminate contamination regularly to protect research results and prevent sample loss. Therefore, customers who buy CO2 incubators are of course concerned about its high reliability, pollution prevention, and ease of use.

CO2 incubator is a high-performance tissue culture incubator with dry heat sterilization function, suitable for life science, clinical medicine, veterinary medicine, pharmaceutical industry, food industry, toxicology, basic biological research and early clinical medical research!
The CO2 incubator is a product suitable for all sensitive cultivation applications. It is suitable for various laboratory applications and provides "more" process control and safety for basic cell culture.
Optional accessories for CO2 incubators , such as oxygen control for creating a hypoxic growth environment, make the CB Series CO2 incubators the ideal equipment choice for custom solutions.
As a precision culture equipment, CO2 incubator requires high CO2 concentration, high temperature, high control precision and small error, which improves the success rate and efficiency of biological cells and tissues. Compared with ordinary incubators, it has irreplaceable advantages.
Why do most cell cultures require a CO2 incubator?
We know that the pH value of general cell culture medium is between 7.0-7.4. Since the carbonate pH buffer system is a physiological pH buffer system (an important pH buffer system in human blood), most cultures use it to maintain a stable pH. When preparing a culture solution with powder, it is often necessary to add A certain amount of sodium bicarbonate.
But for most of the culture fluids that use carbonate as the pH buffer system, in order to maintain a stable pH, the carbon dioxide in the incubator needs to be kept between 2-10% to maintain the concentration of dissolved carbon dioxide in the culture fluid. At the same time, vessels used for cell culture require a certain degree of ventilation to facilitate gas exchange.
So in the case of using other pH buffer systems, is there no need for a CO2 incubator ? Studies have found that due to the low concentration of carbon dioxide in the air, if cells are not cultured in a CO2 incubator, HCO3- will be depleted and affect the cell's normal growth. Therefore, most animal cell cultures still require a CO2 incubator.
It has a strong buffering capacity between pH 7.2-7.4. When the respirable culture vessel is removed from the CO2 incubator, the carbonate buffer system of the culture medium is disabled by the low concentration of carbon dioxide in the air, and the pH value can be kept stable at this time.
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